Purified anti-mouse TCR β chain (Maxpar® -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1272	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed

RRID

AB_2562804 (BioLegend Cat. No. 109235)

Antigen Details

Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution: Majority of T cells and thymocytes (correlated to differentiation)
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound-MHC class I and II
Antigen References: 1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.
Gene ID: 21577 View all products for this Gene ID
UniProt: View information about TCR beta chain on UniProt.org

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