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Pacific Blue™ anti-mouse TCR β chain An
产品名称：
Pacific Blue™ anti-mouse TCR β chain An
产品类别：
抗体
产品编号：
109225
产品应用：
109225
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  2. Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
  3. Westerterp M et al. 2017. Cell metabolism. 25(6):1294-1304 . PubMed
  4. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  5. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  6. Dolina JS, et al. 2020. Cell Rep. 107249:31. PubMed
  7. Gan P, et al. 2017. J Immunol. 10.4049/jimmunol.1602025. PubMed
  8. Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed
  9. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  10. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  11. Pilzecker B, et al. 2016. Nucleic Acids Res. 44: 4734 - 4744. PubMed
  12. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  13. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  14. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  15. Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed
  16. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  17. Dang EV et al. 2017. Cell. 171(5):1057-1071 . PubMed
  18. Redpath S, et al. 2015. Infect Immun . 83: 3881 - 3889. PubMed
  19. Wang X, et al. 2022. Elife. 11:. PubMed
  20. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
RRID
AB_1027655 (BioLegend Cat. No. 109225) AB_1027649 (BioLegend Cat. No. 109226)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

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