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Biotin anti-mouse TCR β chain Antibody
产品名称：
Biotin anti-mouse TCR β chain Antibody
产品类别：
抗体
产品编号：
109203
产品应用：
109203
[价格]
规格 价格 库存
50µg ￥ 648 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. R?dahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  3. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  4. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  5. Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed
  6. Hodes Y 2015. Int Immunol. 27:245. PubMed
  7. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  8. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  9. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  10. Ohta T, et al. 2016. Sci Rep. 6:23505. PubMed
  11. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  12. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  13. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  14. Wada H, et al. 2010. Int Immunol. 1.003472222. PubMed
  15. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  16. Bost P, et al. 2020. Cell. 181(7):1475-1488. PubMed
  17. Bettini M, et al. 2014. J Immunol. 193:258. PubMed
  18. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  19. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  20. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  21. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  22. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  23. Poholek CH, et al. 2019. Immunohorizons. 0.259722222. PubMed
  24. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  25. Sch?fer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  26. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  27. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  28. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID
AB_313426 (BioLegend Cat. No. 109203) AB_313427 (BioLegend Cat. No. 109204)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

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