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Alexa Fluor® 700 anti-mouse TCR β chain A
产品名称：
Alexa Fluor® 700 anti-mouse TCR β chain A
产品类别：
抗体
产品编号：
109223
产品应用：
109223
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Li F, et al. 2022. Mediators Inflamm. 2022:5782922. PubMed
  3. Kim EH, et al. 2020. Elife. 9:00. PubMed
  4. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  5. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  6. Galeano Ni?o JL, et al. 2020. J Cell Sci. . PubMed
  7. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  8. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  9. LaMarche NM, et al. 2020. Cell Metabolism. 32(2):243-258.e6. PubMed
  10. Doisne J, et al. 2009. J Immunol. 183:2142. PubMed
  11. Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed
  12. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
  13. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  14. Kim E, et al. 2022. STAR Protoc. 3:101366. PubMed
  15. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  16. Galeano Ni?o JL, et al. 2020. J Cell Sci. 133:00:00. PubMed
  17. Lebel M?, et al. 2020. Nat Commun. 3.051388889. PubMed
  18. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  19. Elliot TAE, et al. 2021. Immunity. 54:2481. PubMed
  20. Zhu G, et al. 2022. Front Microbiol. 12:806902. PubMed
  21. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  22. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  23. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  24. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  25. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_1027654 (BioLegend Cat. No. 109223) AB_1027648 (BioLegend Cat. No. 109224)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

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