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Alexa Fluor® 647 anti-mouse TCR β chain A
产品名称：
Alexa Fluor® 647 anti-mouse TCR β chain A
产品类别：
抗体
产品编号：
109217
产品应用：
109217
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity purified TCR from mouse DO-11.10 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F -?Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References

(PubMed link indicates BioLegend citation)
  1. Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
  2. Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
  3. Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
  4. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  5. Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
  6. van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
  7. Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
  8. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  9. Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
  12. Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
Product Citations
  1. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  2. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  3. Rowe AM, et al. 2017. J Immunol. 198:1706. PubMed
  4. Rossboth B, et al. 2018. Nat Immunol. 19:821. PubMed
  5. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  6. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  7. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  8. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  9. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  10. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  11. Pageon S, et al. 2016. Proc Natl Acad Sci U S A. 113: E5454 - E5463. PubMed
  12. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  13. Park JY, et al. 2019. Cell Rep. 27:2548. PubMed
  14. Duan H, et al. 2021. J Clin Invest. 131:. PubMed
  15. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  16. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  17. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  18. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  19. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  20. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  21. Mosaheb MM, et al. 2017. Front Immunol. 8:225. PubMed
  22. Werner A, et al. 2021. iScience. 24:103076. PubMed
RRID
AB_493347 (BioLegend Cat. No. 109217) AB_493346 (BioLegend Cat. No. 109218)

Antigen Details

Structure
Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution

Majority of T cells and thymocytes (correlated to differentiation)

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound-MHC class I and II
Antigen References

1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.

Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR beta chain on UniProt.org

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