Alexa Fluor® 594 anti-mouse TCR β chain A-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3036	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Affinity purified TCR from mouse DO-11.10 cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 2-5 μg/ml is suggested. For immunofluorescence microscopy, a concentration range of 2-10 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.?It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

H57-597 is a hamster mAb directed to an epitope of the C region of TCR ? chain¹². The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/? TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation², in vitro stimulation^2,3, in vivo depletion^4-6, and immunohistochemical staining of acetone-fixed frozen sections^7,8,9. The Ultra-LEAF? purified antibody (Endotoxin <0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 109253-109258).

Application References (PubMed link indicates BioLegend citation)

Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.

Product Citations

Li C, et al. 2020. STAR Protoc. 1:100195. PubMed

RRID

AB_2563324 (BioLegend Cat. No. 109238)

Antigen Details

Structure: Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
Distribution: Majority of T cells and thymocytes (correlated to differentiation)
Function: Antigen recognition, T cell activation
Ligand/Receptor: Peptide bound-MHC class I and II
Antigen References: 1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736.
Gene ID: 21577 View all products for this Gene ID
UniProt: View information about TCR beta chain on UniProt.org

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