Purified anti-RNA Polymerase II RPB1 Antibody
- 产品名称：
- Purified anti-RNA Polymerase II RPB1 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 920203
- 产品应用：
- 920203
- Verified Reactivity
- Human, Rat
- Reported Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The antibody H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10.0 ?g/ml. For Western blotting, the use of casein or BSA in the blocking solution rather than non-fat-dry milk is recommended. For immunofluorescence microscopy, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2,3.
The clone H5 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites: ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. Clone H5 recognizes the phosphoserine 2 (ser-2) of Pol II. Clone H5 is useful for immunofluorescence and Western blotting.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
This product weakly reacts with Mouse.
- Application References
(PubMed link indicates BioLegend citation) -
- Brown JM, et al. 2008. J. Cell. Biol. 182:1083. (WB, IF)
- Bregman DB, et al. 1995. Cell Biol. 129:287-98. (IP)
- Ahn SH, et al. 2004. Mol. Cell. 13:67. (IP)
- Pellizzoni L, et al. 2001. Cell Biol. 152(1):75. (IF)
- Patturajan M, et al. 1998. J. Biol. Chem. 273(8):4689. (WB)
- Product Citations
-
- Oqani R, et al. 2016. PLoS One. 11: 0152254. PubMed
- Fitzwalter BE, et al. 2018. Dev Cell. 44:555. PubMed
- Butler AM, et al. 2018. Development. 145:. PubMed
- Wong MM et al. 2018. Developmental cell. 46(3):302-315 . PubMed
- Yurko N, et al. 2017. Mol Cell. 68:913. PubMed
- Li Y et al. 2017. Development (Cambridge, England). 144(18):3232-3240 . PubMed
- Montinaro A, et al. 2021. Cell Death Differ. Online ahead of print. PubMed
- Yamashita T, et al. 2017. Sci Rep. 7:39994. PubMed
- Ryu HY, et al. 2019. EMBO J. 38:e102003. PubMed
- Baluapuri A, et al. 2019. Mol Cell. 74:674. PubMed
- Kuhn TM, et al. 2019. J Cell Biol. 218:2945. PubMed
- Zhou KI, et al. 2019. Mol Cell. 76:70. PubMed
- Tolza C, et al. 2019. Mol Cancer Res. 17:1999. PubMed
- Rona G et al. 2018. eLife. 7 pii: e38771. PubMed
- Chappidi N, et al. 2020. Molecular Cell. 77(3):528-541.e8.. PubMed
- Zhang H et al. 2018. Cell. 175(5):1244-1258 . PubMed
- Stanek TJ, et al. 2021. EMBO J. 40:e102509. PubMed
- Pancholi A, et al. 2021. Mol Syst Biol. 17:e10272. PubMed
- RRID
- AB_2734687 (BioLegend Cat. No. 920203) AB_2616695 (BioLegend Cat. No. 920204)
- Structure
- RPB1 contains a C-terminus composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain Ser and Thr residues that are phosphorylated in actively transcribing RNA polymerase. The predicted molecular weight is 220 kD.
- Distribution
-
Nucleus and cytosol.
- Function
- RPB1 is the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Gene ID
- 5430 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase II on UniProt.org
