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Purified anti-human CD107b (LAMP-2) Antibody
产品名称:
Purified anti-human CD107b (LAMP-2) Antibody
产品类别:
抗体
产品编号:
354301
产品应用:
354301
[价格]
规格 价格 库存
25µg ¥ 708 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Adult human adherent spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
IHC-P, ICC, WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. For Western blotting, the suggested use of this reagent is 1.0?- 5.0??g per ml. For immunocytochemistry, a concentration range of 5.0?- 10 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of ?neutrophils2,3.

This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
  2. Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
  3. Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
Product Citations
  1. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  2. de Leeuw SM, et al. 2022. Stem Cell Reports. 17:110. PubMed
  3. Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
  4. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  5. Bassaganyas L, et al. 2019. J Cell Biol. 218:3861. PubMed
RRID
AB_11204081 (BioLegend Cat. No. 354301) AB_11204245 (BioLegend Cat. No. 354302)

Antigen Details

Structure
LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
Distribution

Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines

Function
Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
Cell Type
B cells, Lymphocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Chen J,?et al.?1985.?J. Biol. Chem.?101:85.
2. Kain R,?et al.?2008.?Nat. Med. 14:1088.
3. Roark EA,?et al.?2008.?PLoS ONE?3:e3538.

Gene ID
3920 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org

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