Purified anti-human CD107b (LAMP-2) Antibody
- 产品名称:
- Purified anti-human CD107b (LAMP-2) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 354301
- 产品应用:
- 354301
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Adult human adherent spleen cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
IHC-P, ICC, WB - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 ?g per ml. For Western blotting, the suggested use of this reagent is 1.0?- 5.0??g per ml. For immunocytochemistry, a concentration range of 5.0?- 10 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of ?neutrophils2,3.
This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
- Application References
(PubMed link indicates BioLegend citation) -
- Chen J, et al. 1985. J. Biol. Chem. 101:85.
- Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
- Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
- Product Citations
-
- Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
- de Leeuw SM, et al. 2022. Stem Cell Reports. 17:110. PubMed
- Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
- Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
- Bassaganyas L, et al. 2019. J Cell Biol. 218:3861. PubMed
- RRID
- AB_11204081 (BioLegend Cat. No. 354301) AB_11204245 (BioLegend Cat. No. 354302)
- Structure
- LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
- Distribution
-
Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines
- Function
- Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
- Cell Type
- B cells, Lymphocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Chen J,?et al.?1985.?J. Biol. Chem.?101:85.
2. Kain R,?et al.?2008.?Nat. Med. 14:1088.
3. Roark EA,?et al.?2008.?PLoS ONE?3:e3538.
- Gene ID
- 3920 View all products for this Gene ID
- UniProt
- View information about CD107b on UniProt.org