Alexa Fluor® 647 anti-human CD107b (LAMP-2) An
- 产品名称：
- Alexa Fluor® 647 anti-human CD107b (LAMP-2) An
- 产品类别：
- 抗体
- 产品编号：
- 354311
- 产品应用：
- 354311
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1344 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Adult human adherent spleen cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC -?Quality tested
IHC-P -?Verified?
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemistry, a concentration of 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of ?neutrophils2,3.
This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.
- Application References
(PubMed link indicates BioLegend citation) -
- Chen J, et al. 1985. J. Biol. Chem. 101:85.
- Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
- Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
- Product Citations
-
- Chin MY, et al. 2021. ACS Sens. 6:2168. PubMed
- RRID
- AB_2721404 (BioLegend Cat. No. 354311) AB_2721405 (BioLegend Cat. No. 354312)
- Structure
- LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
- Distribution
-
Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines
- Function
- Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
- Cell Type
- B cells, Lymphocytes, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Chen J,?et al.?1985.?J. Biol. Chem.?101:85.
2. Kain R,?et al.?2008.?Nat. Med. 14:1088.
3. Roark EA,?et al.?2008.?PLoS ONE?3:e3538.
- Gene ID
- 3920 View all products for this Gene ID
- UniProt
- View information about CD107b on UniProt.org
