PE/Cyanine5 anti-human CD107a (LAMP-1) Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 1176	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human adult adherent peripheral blood cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting⁸, immunohistochemical staining², immunofluorescence^5,7, and immunoprecipitation⁵.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References (PubMed link indicates BioLegend citation)

Misse D, et al. 1999. Blood 93:2454.
Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
Salio M, et al. 2013 PNAS. 110:4753. PubMed

RRID

AB_2910392 (BioLegend Cat. No. 328655)

Antigen Details

Structure: LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution: Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes
Function: Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor: Galaptin
Cell Type: Endothelial cells, Epithelial cells, Macrophages
Biology Area: Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.
Gene ID: 3916 View all products for this Gene ID
UniProt: View information about CD107a on UniProt.org

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