Biotin anti-human CD218a (IL-18Rα) Antibody
- 产品名称：
- Biotin anti-human CD218a (IL-18Rα) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 313806
- 产品应用：
- 313806
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NK cell line NK0 constitutively expressing IL-18 receptors
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
?The H44 antibody is specific for IL-18 receptor a chain. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and neutralization1.
- Application References
(PubMed link indicates BioLegend citation) -
- Kitasato Y, et al. 2004. Am. J. Respir. Cell Mol. Biol. 31:619. (IHC)
- Vermot-Desroches C, et al. 2005. Cell Immunol. 236:101. (FC)
- Product Citations
-
- Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
- RRID
- AB_2123649 (BioLegend Cat. No. 313806)
- Structure
- Type I membrane protein, IL-1 receptor family, approximately 75-80 kD
- Distribution
-
Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines
- Function
- Binds IL-18 with low affinity; high affinity binding site formed with IL-18Rβ
- Ligand/Receptor
- IL-18
- Cell Type
- B cells, Endothelial cells, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Torigoe K, et al. 1997. J. Biol. Chem. 272:25737.
2. Gerdes N, et al. 2002. J. Exp. Med. 195:245.
3. Airoldi I, et al. 2000. J. Immunol. 165:6880.
- Gene ID
- 8809 View all products for this Gene ID
- UniProt
- View information about CD218a on UniProt.org
