Ultra-LEAF™ Purified anti-mouse IFN-γ -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1320	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Recombinant full-length murine IFN-γ

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

WB - Quality tested
Neut, ELISA, ICC, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 ?g per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified H22 antibody is useful in mouse IFN-γ ELISA, immunofluorescence and immunoprecipitation applications. For in vitro or in vivo neutralization applications, it is suggested to use LEAF? Purified H22. It is recommended that the reagent be titrated for optimal performance for each application.

Application References (PubMed link indicates BioLegend citation)

Schreiber RD, et al. 1985. J. Immunol. 134:1609.
Williams RO, et al. 1993. Clin. Exp. Immunol. 92:323
Steed AL, et al. 2006. J. Virol. 80:192.
Wu LL, et al. 2014. Am J Pathol. 184:2260. PubMed

RRID

AB_2810645 (BioLegend Cat. No. 513207) AB_2810646 (BioLegend Cat. No. 513208) AB_2810647 (BioLegend Cat. No. 513209) AB_2810648 (BioLegend Cat. No. 513210) AB_2810649 (BioLegend Cat. No. 513211) AB_2810650 (BioLegend Cat. No. 513212)

Antigen Details

Structure: Cytokine; dimer; 40-80 kD (Mammalian)
Function: Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Interaction: T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Ligand/Receptor: IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Bioactivity: Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources: CD8⁺ and CD4⁺ T cells, NK cells
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571
4. Gray P, et al. 1987. Lymphokines 13:151
Gene ID: 15978 View all products for this Gene ID
UniProt: View information about IFN-gamma on UniProt.org

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