PerCP/Cyanine5.5 anti-mouse CD11a/CD18 (LFA-1) Ant
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD11a/CD18 (LFA-1) Ant
- 产品类别：
- 抗体
- 产品编号：
- 141007
- 产品应用：
- 141007
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TH-derived anti-I-Ak CTL clone A1 5.1.17
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding1.
- Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Pont S, et al. 1986. J. Immunol. 136:3750. (FC)
- Product Citations
-
- Dupont C, et al. 2015. J Immunol. 195: 4369 - 4377. PubMed
- Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
- Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
- Markey K, et al. 2014. J Immunol. 192:5426. PubMed
- Dupont C, et al. 2014. PLoS Pathog. 10:1004047. PubMed
- Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
- Markey KA et al. 2019. Current protocols in immunology. 125(1):e72 . PubMed
- Czupalla CJ, et al. 2018. Bio Protoc. 8:. PubMed
- RRID
- AB_10694861 (BioLegend Cat. No. 141007) AB_10694862 (BioLegend Cat. No. 141008)
- Structure
- Heterodimer composed of two members of the integrin family, &alphaL (CD11a) and β2 (CD18).
- Distribution
-
Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes
- Function
- Intercellular adhesion and costimulation
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
- Cell Type
- B cells, Dendritic cells, Lymphocytes, Macrophages, Monocytes
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22.
2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
3. Springer TA. 1994. Cell 76:301.
4. Lub M, et al. 1995. Immunol. Today 16:479.
- Gene ID
- 16408 View all products for this Gene ID 16414 View all products for this Gene ID
- UniProt
- View information about CD11a CD18 on UniProt.org
