Pacific Blue™ anti-mouse CD11a/CD18 (LFA-1)
- 产品名称：
- Pacific Blue™ anti-mouse CD11a/CD18 (LFA-1)
- 产品类别：
- 抗体
- 产品编号：
- 141013
- 产品应用：
- 141013
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TH-derived anti-I-Ak CTL clone A1 5.1.17
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding1.
- Application References
(PubMed link indicates BioLegend citation) -
- Pont S, et al. 1986. J. Immunol. 136:3750. (FC)
- RRID
- AB_2800652 (BioLegend Cat. No. 141013) AB_2800653 (BioLegend Cat. No. 141014)
- Structure
- Heterodimer composed of two members of the integrin family, &alphaL (CD11a) and β2 (CD18).
- Distribution
-
Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes
- Function
- Intercellular adhesion and costimulation
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
- Cell Type
- B cells, Dendritic cells, Lymphocytes, Macrophages, Monocytes
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22.
2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
3. Springer TA. 1994. Cell 76:301.
4. Lub M, et al. 1995. Immunol. Today 16:479.
- Gene ID
- 16408 View all products for this Gene ID 16414 View all products for this Gene ID
- UniProt
- View information about CD11a CD18 on UniProt.org
