APC anti-mouse CD11a/CD18 (LFA-1) Antibody
- 产品名称：
- APC anti-mouse CD11a/CD18 (LFA-1) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 141009
- 产品应用：
- 141009
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TH-derived anti-I-Ak CTL clone A1 5.1.17
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
This clone is reported to have some effect on increasing LFA-1-mediated adhesion and does not block LFA-1 binding1.
- Application References
(PubMed link indicates BioLegend citation) -
- Pont S, et al. 1986. J. Immunol. 136:3750. (FC)
- Product Citations
-
- Kim HR, et al. 2018. Nat Commun. 9:3630. PubMed
- Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
- Girón-Pérez DA, et al. 2020. J Cell Sci. 133:00:00. PubMed
- Crinier A, et al. 2018. Immunity. 49:971. PubMed
- RRID
- AB_2564305 (BioLegend Cat. No. 141009) AB_2564306 (BioLegend Cat. No. 141010)
- Structure
- Heterodimer composed of two members of the integrin family, &alphaL (CD11a) and β2 (CD18).
- Distribution
-
Primarily expressed on lymphocytes, monocytes/macrophages, and granulocytes
- Function
- Intercellular adhesion and costimulation
- Ligand/Receptor
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), and ICAM-3 (CD242)
- Cell Type
- B cells, Dendritic cells, Lymphocytes, Macrophages, Monocytes
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Dugger KJ, et al. 2009. J. Neuroimmunol. 206:22.
2. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
3. Springer TA. 1994. Cell 76:301.
4. Lub M, et al. 1995. Immunol. Today 16:479.
- Gene ID
- 16408 View all products for this Gene ID 16414 View all products for this Gene ID
- UniProt
- View information about CD11a CD18 on UniProt.org
