Direct-Blot™ HRP anti-RNA Polymerase II RPB1
- 产品名称：
- Direct-Blot™ HRP anti-RNA Polymerase II RPB1
- 产品类别：
- 抗体
- 产品编号：
- 920305
- 产品应用：
- 920305
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 0.1 ?g per ml (1:5000 - 1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2,3.
The clone H14 was developed against a purified phosphorylated form of RNA polymerase II extracted from a transformed cell line. Pol II has two physiologically important phosphorylation sites: ser-2 and ser-5. Both sites are found in the heptapeptide repeat YSPTSPS at the C-terminal domain. Clone H14 recognizes the phosphoserine 5 (ser-5) of Pol II. Clone H14 is useful for immunofluorescence and Western blotting.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- Application References
(PubMed link indicates BioLegend citation) -
- Brown JM, et al. 2008. J. Cell. Biol. 182:1083. (WB, IF)
- Bregman DB, et al. 1995. Cell Biol. 129:287-98. (IP)
- Ahn SH, et al. 2004. Mol. Cell. 13:67. (IP)
- Pellizzoni L, et al. 2001. Cell Biol. 152(1):75. (IF)
- Patturajan M, et al. 1998. J. Biol. Chem. 273(8):4689. (WB)
- RRID
- AB_2734688 (BioLegend Cat. No. 920305) AB_2734689 (BioLegend Cat. No. 920306)
- Structure
- RPB1 contains a C-terminus composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain Ser and Thr residues that are phosphorylated in actively transcribing RNA polymerase. The predicted molecular weight is 220 kD.
- Distribution
-
Nucleus and cytosol.
- Function
- RPB1 is the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 5430 View all products for this Gene ID
- UniProt
- View information about RNA Polymerase II RPB1 on UniProt.org
