PE/Cyanine5 anti-mouse CD4 Antibody
- 产品名称：
- PE/Cyanine5 anti-mouse CD4 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 130312
- 产品应用：
- 130312
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1-3, depletion. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Naquet P. 1985. Immunogenetics. 22:247-256.
- Pierres A. 1984. J. Immunol.. 132:2775-2782.
- Guo MW. 1995. Zygote. 3:65-73.
- Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506.
- Product Citations
-
- Pan H, et al. 2020. Mol Psychiatry. . PubMed
- Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
- RRID
- AB_2075572 (BioLegend Cat. No. 130312)
- Structure
- Ig superfamily, 55 kD
- Distribution
- Majority of thymocytes, T cell subset
- Function
- TCR co-receptor, T cell activation
- Ligand/Receptor
- MHC class II molecule
- Cell Type
- Thymocytes, T cells, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
- 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.
4. Naquet P. 1985. Immunogenetics. 22:247-256.
5. Pierres A. 1984. J. Immunol.. 132:2775-2782.
6. Guo MW. 1995. Zygote. 3:65-73.
7. Ghobrial RR. 1989. Clin Immunol Immunopathol. 52:486-506. - Gene ID
- 12504 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
