Purified anti-Myosin Heavy Chain Antibody
- 产品名称：
- Purified anti-Myosin Heavy Chain Antibody
- 产品类别：
- 抗体
- 产品编号：
- 922703
- 产品应用：
- 922703
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was generated against a complex mixture of phosphoproteins extracted from Madin-Darby Canine Kidney Cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25?- 1.0??g/mL. For immunocytochemistry, a concentration?of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
When using this clone for ICC, we recommend fixing cells with 4% PFA and permeabilizing with methanol. The clone was not compatible with mysosin heavy chain staining when cells were fixed/permeabilized with methanol only or 4% PFA + Triton X-100.
- RRID
- AB_2894519 (BioLegend Cat. No. 922703) AB_2894519 (BioLegend Cat. No. 922704)
- Structure
- Actin-based motor protein, approximately 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment).
- Distribution
-
Highly expressed in muscle.
- Function
- Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion/ Cysteine residues in the S1 domain are alkylated for myosin ATPase activity
- Interaction
- Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits. Forms filaments.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Weiss A, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2598.
- Schiaffino S, et al. 1996. Physiol. Rev. 76:371
- Gene ID
- NA
- UniProt
- View information about Myosin Heavy Chain on UniProt.org
