Anti-Myosin Heavy Chain Antibody (Previously Covan
- 产品名称：
- Anti-Myosin Heavy Chain Antibody (Previously Covan
- 产品类别：
- 抗体
- 产品编号：
- 922701
- 产品应用：
- 922701
- Verified Reactivity
- Mammalian
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was generated against a complex mixture of phosphoproteins extracted from Madin-Darby Canine Kidney Cells.
- Preparation
- Ascites
- Concentration
- The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
-
WB -?Quality tested
ICC -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 - 1:2500. For immunocytochemistry, a dilution of 1:1000 - 1:250 is recommended.?For immunoprecipitation, the suggested use of this reagent is a dilution of 1:150.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody is effective in immunoblotting (WB), immunofluorescence (ICC) and immunoprecipitation (IP).
*Predicted MW = 205 kD
**Recommend using extracts prepared in buffers containing 0.5% Triton X-100 and 0.5% sodium deoxycholate.
This antibody binds specifically to denatured as well as native myosin heavy chain extracts and will not react with amphibian myosins.?
- Application References
(PubMed link indicates BioLegend citation) -
- Chew TL, et al. 2002. J. Cell Biol. 156:543. (ICC) PubMed
- Warren SL, et al. 1988. Mol Cell Biol. 8:632.
- Product Citations
-
- Chew T, et al. 2002. J Cell Biol. 156:543-553. PubMed
- Aaron JS, et al. 2018. J Cell Sci. 131. PubMed
- Zehrer A, et al. 2018. J Immunol. 201:1748. PubMed
- RRID
- AB_2565417 (BioLegend Cat. No. 922701)
- Structure
- Actin-based motor protein, approximately 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment).
- Distribution
-
Highly expressed in muscle.
- Function
- Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion.
- Interaction
- Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits. Forms filaments.
- Modification
- Alkylation.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
1. Weiss A, et al. 1999. P. Natl. Acad. Sci. USA 96:2598.
2. Schiaffino S, et al. 1996. Physiol. Rev. 76:371 (review)
- Regulation
- Cysteine residues in the S1 domain are alkylated for myosin ATPase activity.
- Gene ID
- NA
- UniProt
- View information about Myosin Heavy Chain on UniProt.org
