Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation^1-3, immunohistochemistry^4,5, and immunoprecipitation¹.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.

The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Scientific Poster Library.

1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
2. Sobel ES, et al. 1993. J. Immunol. 150:673.
3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
7. Lee JW, et al. 2006. Nature Immunol. 8:181.
8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
9. Jordan JM, et al. 2008. 76:3717. PubMed
10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.