Purified anti-mouse CD69 Antibody
- 产品名称：
- Purified anti-mouse CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 104502
- 产品应用：
- 104502
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse dendritic epidermal T cell line Y245
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.
This antibody has been characterized in the literature as containing a lambda (?) light chain.
- Application References
(PubMed link indicates BioLegend citation) -
- Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
- Sobel ES, et al. 1993. J. Immunol. 150:673.
- Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
- Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Lee JW, et al. 2006. Nature Immunol. 8:181.
- Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
- Jordan JM, et al. 2008. 76:3717. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
- Product Citations
-
- Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
- Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
- Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
- Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
- Guo R, et al. 2020. Cell Res. 30:21. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
- Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
- Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
- Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
- Oda SK, et al. 2020. J Exp Med. 217: . PubMed
- Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
- Kroczek AL, et al. 2019. Front Immunol. 9:2806. PubMed
- Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
- Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
- Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
- Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
- Kiripolsky J, et al. 2021. J Autoimmun. 118:102608. PubMed
- Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
- Snell LM, et al. 2018. Immunity. 49:678. PubMed
- Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
- Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
- Liu H, et al. 2020. J Immunol. 205:1207. PubMed
- Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
- Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
- Hsiao CC, et al. 2021. Cells. 10:. PubMed
- Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
- Park GY, et al. 2020. Immune Netw. e43:20. PubMed
- Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
- Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
- Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
- Kenna T, et al. 2008. Blood. 111:2091. PubMed
- Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
- Karmaus P, et al. 2011. J Leukoc Biol. 90:983. PubMed
- Roy S, et al. 2018. Cancer Res. 78:5600. PubMed
- Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
- Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
- Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
- Cui X, et al. 2017. J Immunol. 199:4066. PubMed
- Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
- Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
- Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
- Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
- Richards KA, et al. 2018. Front Immunol. 0.829861111. PubMed
- Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
- Diao J, et al. 2018. J Immunol. 201:1306. PubMed
- Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
- Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
- Epardaud M, et al. 2008. Cancer Res. 68:2972. PubMed
- K?stele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
- Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
- RRID
- AB_313105 (BioLegend Cat. No. 104502)
- Structure
- C-type lectin, 27/33 kD
- Distribution
-
Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets
- Function
- Lymphocyte activation
- Cell Type
- B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.
- Gene ID
- 12515 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
