PerCP/Cyanine5.5 anti-mouse CD69 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 104521
- 产品应用：
- 104521
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse dendritic epidermal T cell line Y245
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per 106 cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.
This antibody has been characterized in the literature as containing a lambda (?) light chain.
- Additional Product Notes
View more applications data for this product in our Scientific Poster Library.
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
- Sobel ES, et al. 1993. J. Immunol. 150:673.
- Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
- Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Lee JW, et al. 2006. Nature Immunol. 8:181.
- Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
- Jordan JM, et al. 2008. 76:3717. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
- Product Citations
-
- Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
- Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
- Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
- Tan L, et al. 2019. Cell Rep. 27:3657. PubMed
- Lutes LK, et al. 2021. eLife. 10:00. PubMed
- Nijnik A, et al. 2009. J Immunol. 183:5788. PubMed
- Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
- Amor C, et al. 2020. Nature. 583:127. PubMed
- Soni C, et al. 2014. J Immunol. 193:4400. PubMed
- Schofield DJ, et al. 2021. MAbs. 13:1857100. PubMed
- Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
- Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
- Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
- Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
- Yu X, et al. 2019. Nat Commun. 10:574. PubMed
- Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
- Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
- Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
- Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
- Yuan X, et al. 2017. Elife. 6:e29540. PubMed
- Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
- Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
- Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
- Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
- Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
- Tomaru U et al. 2019. Cell reports. 26(3):639-651 . PubMed
- Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed
- RRID
- AB_940497 (BioLegend Cat. No. 104521) AB_2260065 (BioLegend Cat. No. 104522)
- Structure
- C-type lectin, 27/33 kD
- Distribution
-
Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets
- Function
- Lymphocyte activation
- Cell Type
- B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.
- Gene ID
- 12515 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
