APC anti-mouse CD69 Antibody
- 产品名称:
- APC anti-mouse CD69 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 104513
- 产品应用:
- 104513
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse dendritic epidermal T cell line Y245
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.
This antibody has been characterized in the literature as containing a lambda (?) light chain.
- Application References
(PubMed link indicates BioLegend citation) -
- Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
- Sobel ES, et al. 1993. J. Immunol. 150:673.
- Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
- Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Lee JW, et al. 2006. Nature Immunol. 8:181.
- Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
- Jordan JM, et al. 2008. 76:3717. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
- Product Citations
-
- Stepanek O, et al. 2013. J Immunol. 190:1807. PubMed
- Kongsomboonvech AK, et al. 2020. PLoS Pathog. 16:e1008327. PubMed
- Su Y, et al. 2022. J Hematol Oncol. 15:99. PubMed
- Michelet X, et al. 2015. J Immunol. 194:2079. PubMed
- Català C, et al. 2022. iScience. 25:105078. PubMed
- Files M, et al. 2022. NPJ Vaccines. 7:48. PubMed
- Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
- Diao L, et al. 2022. iScience. 25:105511. PubMed
- del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
- Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
- Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
- Wang Z, et al. 2021. J Immunother Cancer. 9: . PubMed
- Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
- Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
- Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
- Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
- Wang Z, et al. 2019. J Clin Invest. 130:4850. PubMed
- Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
- Woyciechowski S, Hofmann M, Pircher H 2017. Eur J Immunol. 47:244-250. PubMed
- Dolina JS, et al. 2020. Cell Rep. 107249:31. PubMed
- Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
- Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
- Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
- MacDonald A, et al. 2021. Front Immunol. 12:755995. PubMed
- Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
- Lee J, et al. 2022. Clin Transl Med. 12:e1021. PubMed
- Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
- Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
- Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
- Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
- Xu L, et al. 2012. J Immunol. 188:248. PubMed
- Noviski M, et al. 2018. Elife. 7:e35074. PubMed
- LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
- Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
- Kim S, et al. 2021. Elife. 10:. PubMed
- Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
- Morrison V, et al. 2015. J Immunol. 195: 105 - 115. PubMed
- Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
- Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
- Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
- Xie X, et al. 2009. J Immunol. 182:7163. PubMed
- Lebrun A, et al. 2015. J Immunol. 195: 4358 - 4368. PubMed
- Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
- Li J, et al. 2020. Development. . PubMed
- Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
- Okuma A, et al. 2021. Methods Mol Biol. 2312:3. PubMed
- Elliot TAE, et al. 2021. Immunity. 54:2481. PubMed
- Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
- Yan C, et al. 2022. NPJ Precis Oncol. 6:6. PubMed
- Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
- Xu Y, et al. 2021. iScience. 24:103445. PubMed
- Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
- Sun Y, et al. 2022. iScience. 25:104846. PubMed
- Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
- Lin Y, et al. 2012. Cancer Prev Res. 5:1090. PubMed
- Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
- RRID
- AB_492844 (BioLegend Cat. No. 104513) AB_492843 (BioLegend Cat. No. 104514)
- Structure
- C-type lectin, 27/33 kD
- Distribution
-
Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets
- Function
- Lymphocyte activation
- Cell Type
- B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.
- Gene ID
- 12515 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org