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Brilliant Violet 421™ anti-human/mouse CD49f
产品名称：
Brilliant Violet 421™ anti-human/mouse CD49f
产品类别：
抗体
产品编号：
313623
产品应用：
313623
[价格]
规格 价格 库存
25tests ￥ 2376 1

产品详情

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Mouse, Baboon, Capuchin Monkey, Cat, Cow, Chimpanzee, Cynomolgus, Dog, Horse, Rabbit, Sheep, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemistry, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.

Application References

(PubMed link indicates BioLegend citation)
  1. Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
  2. Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
  3. Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
  4. Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
  5. Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
  6. Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
  7. Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
  8. Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
  9. Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
  10. Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
  11. Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
  14. Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
  15. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Product Citations
  1. Moriyama M, et al. 2016. PLoS One. 11:e0164799. PubMed
  2. Yamashiro C, et al. 2020. Nat Protoc. 15:1560. PubMed
  3. Bach K, et al. 2021. Nat Commun. 1502:12. PubMed
  4. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  5. Mok KW et al. 2018. Developmental cell. 48(1):32-48 . PubMed
  6. Chen D, et al. 2019. Cell Rep. 29:4568. PubMed
  7. Murase Y, et al. 2020. EMBO J. 39:e104929. PubMed
  8. Gell JJ, et al. 2018. Stem Cell Res. 27:46. PubMed
  9. Io S, et al. 2021. Cell Stem Cell. 28(6):1023-1039.e13. PubMed
  10. Lau KX, et al. 2020. Nat Commun. 11:2420. PubMed
  11. Hancock GV, et al. 2021. Stem Cell Res. 55:102493. PubMed
  12. Yamashiro C, et al. 2020. Nature Protocols. 362(6412):356-360. PubMed
  13. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  14. Chen D et al. 2018. Cell reports. 25(13):3591-3602 . PubMed
  15. Kojima Y et al. 2017. Cell stem cell. 21(4):517-532 . PubMed
  16. Xiang X, et al. 2022. Nat Commun. 13:463. PubMed
  17. Chen D, et al. 2017. Biol Reprod. 97:850. PubMed
  18. Sosa E, et al. 2018. Nat Commun. 9:5339. PubMed
  19. Yuan W, et al. 2021. Int J Biol Sci. 17:527. PubMed
  20. Chitiashvili T, et al. 2020. Nat Cell Biol. 22:1436. PubMed
  21. Pandolfi EC, et al. 2022. Cell Rep Med. 3:100782. PubMed
RRID
AB_2562243 (BioLegend Cat. No. 313623) AB_2562244 (BioLegend Cat. No. 313624)

Antigen Details

Structure
Integrin family, associates with β1 or β4, 120 kD
Distribution

Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells

Function
Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
Ligand/Receptor
With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360.

Gene ID
16403 View all products for this Gene ID 3655 View all products for this Gene ID
UniProt
View information about CD49f on UniProt.org

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