Purified anti-Glu-Glu Epitope Tag Antibody (Previo
- 产品名称：
- Purified anti-Glu-Glu Epitope Tag Antibody (Previo
- 产品类别：
- 抗体
- 产品编号：
- 901801
- 产品应用：
- 901801
- Verified Reactivity
- EYMPME and EFMPME Tags
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The Glu-Glu monoclonal antibody was raised against the peptide sequence CEEEEYMPME derived from the polyoma virus medium T antigen.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
IP, ICC, Purification
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. BioLegend antibodies may not be resold or modified for resale without prior written approval.
? WB: 1:1,000*
? IF: 1:1,000
? IP: 1:150
- Application Notes
This antibody is effective in immunofluorescence, immunoblotting, and immunoprecipitation of tagged proteins.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
It is extremely specific and recognizes either six amino acid sequence, EYMPME, or EFMPME.
- Application References
(PubMed link indicates BioLegend citation) -
- Wells CD, et al. 2001. J. Biol. Chem. 276:28897.
- Kim M, et al. 2001. J. Biol. Chem. 276:23262.
- Grussenmeyer T, et al. 1985. Proc. Nat. Acad. Sci. USA. 82:7952.
- Pinkas J, et al. 2002. Cancer Res. 62:4781. (WB, IF) PubMed
- Jeon JP, et al. 2012. J. Biol. Chem. 287:17029. (WB)
- Product Citations
-
- Maziarz M, et al. 2020. J Biol Chem. 295:16897. PubMed
- Maziarz M, et al. 2020. J Biol Chem. 295:16897. PubMed
- Leder J 2002. Cancer Res. 62:4781-4790. PubMed
- Smolko CM, et al. 2019. Sci Rep. 9:19409. PubMed
- Onken MD, et al. 2018. Sci Signal. 11. PubMed
- Hoy JJ, et al. 2020. FASEB J. 34:13900. PubMed
- RRID
- AB_2565011 (BioLegend Cat. No. 901801) AB_2565012 (BioLegend Cat. No. 901802)
