Purified anti-mouse/human GL7 Antigen (T and B cel
- 产品名称：
- Purified anti-mouse/human GL7 Antigen (T and B cel
- 产品类别：
- 抗体
- 产品编号：
- 144601
- 产品应用：
- 144601
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ELISA, IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 ?g per ml.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
- Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
- Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
- Fonseca JA, et al. 2018. Vaccine. 36:2799. PubMed
- Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
- Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed
- Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
- Mathur S, et al. 2021. JCI Insight. 6:. PubMed
- Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
- Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
- Firmino NS, et al. 2021. Oncoimmunology. 10:1959978. PubMed
- Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
- Papa I, et al. 2017. Nature. 547:318. PubMed
- Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
- Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
- RRID
- AB_2561547 (BioLegend Cat. No. 144601) AB_2561548 (BioLegend Cat. No. 144602)
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand/Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.
- Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
