PerCP/Cyanine5.5 anti-mouse/human GL7 Antigen (T a
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse/human GL7 Antigen (T a
- 产品类别：
- 抗体
- 产品编号：
- 144609
- 产品应用：
- 144609
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
- Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
- Suzuki Y, et al. 2022. Mol Ther Nucleic Acids. 30:226. PubMed
- Mathur S, et al. 2021. JCI Insight. 6:. PubMed
- Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
- Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
- Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
- Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
- Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
- Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Gary E, et al. 2020. Vaccine. 3821:38. PubMed
- Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
- Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
- Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
- Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
- Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
- Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
- Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
- Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
- Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
- Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
- Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
- Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
- Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
- Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
- Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
- RRID
- AB_2562978 (BioLegend Cat. No. 144609) AB_2562979 (BioLegend Cat. No. 144610)
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand/Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.
- Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
