PE anti-mouse/human GL7 Antigen (T and B cell Acti
- 产品名称：
- PE anti-mouse/human GL7 Antigen (T and B cell Acti
- 产品类别：
- 抗体
- 产品编号：
- 144607
- 产品应用：
- 144607
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
- Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- Jing L, et al. 2022. Front Immunol. 13:864995. PubMed
- Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
- Kania AK, et al. 2021. Immunohorizons. 5:918. PubMed
- Abbott RK, et al. 2018. Immunity. 48:133. PubMed
- Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
- He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
- Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
- Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
- Chen RJ, et al. 2022. iScience. 25:105595. PubMed
- Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
- Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
- Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
- Andersen TK, et al. 2021. Front Immunol. 12:747032. PubMed
- Vijay R, et al. 2021. J Exp Med. 218:. PubMed
- Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
- Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
- Ryg-Cornejo V, et al. 2016. Cell Rep. 14:68-81. PubMed
- Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
- Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
- RRID
- AB_2562925 (BioLegend Cat. No. 144607) AB_2562926 (BioLegend Cat. No. 144608)
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand/Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.
- Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
