Pacific Blue™ anti-mouse/human GL7 Antigen (
- 产品名称：
- Pacific Blue™ anti-mouse/human GL7 Antigen (
- 产品类别：
- 抗体
- 产品编号：
- 144613
- 产品应用：
- 144613
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
- Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
- Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
- Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
- Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
- Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
- Huang L, et al. 2017. PLoS Biol.. 10.1371/journal.pbio.2001750. PubMed
- Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
- Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed
- Duan L, et al. 2021. Immunity. 54:2256. PubMed
- Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed
- Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
- Mintz MA, et al. 2019. Immunity. 51:310. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
- Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
- Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
- Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
- Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
- Zhou X, et al. 2021. Cell Reports. 34(1):108601. PubMed
- Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
- Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
- Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
- van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
- Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
- Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
- Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
- RRID
- AB_2563291 (BioLegend Cat. No. 144613) AB_2563292 (BioLegend Cat. No. 144614)
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand/Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.
- Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
