Alexa Fluor® 647 anti-mouse/human GL7 Antigen
- 产品名称：
- Alexa Fluor® 647 anti-mouse/human GL7 Antigen
- 产品类别：
- 抗体
- 产品编号：
- 144605
- 产品应用：
- 144605
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume.?For immunohistochemistry, a concentration range of 2.5 - 5 ?g/ml is suggested.?It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
- Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- Zhao J, et al. 2021. Emerg Microbes Infect. 10:913. PubMed
- Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
- Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
- Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
- Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
- Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
- Essig K et al. 2017. Immunity. 47(6):1067-1082 . PubMed
- Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
- Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
- Zanotti KJ, et al. 2019. J Immunol. 202:1573. PubMed
- Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
- Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
- Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
- Ise W, et al. 2018. Immunity. 48:702. PubMed
- Glaros V, et al. 2021. Immunity. 54:2005. PubMed
- Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
- Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
- Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
- Castiblanco D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01833. PubMed
- Ullrich L, et al. 2021. Front Immunol. 12:729607. PubMed
- Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
- Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
- Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
- Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
- Maul R, et al. 2016. J Exp Med. 213: 1675 - 1683. PubMed
- Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
- Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
- Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
- Wu S, et al. 2016. Proc Natl Acad Sci U S A. 113: 9816 - 9821. PubMed
- Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
- Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
- Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
- Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
- Kleiman E, et al. 2016. Proc Natl Acad Sci U S A. 113: E3911 - E3920. PubMed
- Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
- Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
- Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
- RRID
- AB_2562184 (BioLegend Cat. No. 144605) AB_2562185 (BioLegend Cat. No. 144606)
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand/Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.
- Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
