Alexa Fluor® 488 anti-mouse/human GL7 Antigen
- 产品名称：
- Alexa Fluor® 488 anti-mouse/human GL7 Antigen
- 产品类别：
- 抗体
- 产品编号：
- 144611
- 产品应用：
- 144611
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- LPS activated DBA/J mouse B cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume.?For immunohistochemistry, a concentration range of 2.5 - 5 ?g/ml is suggested.?It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
- Application References
(PubMed link indicates BioLegend citation) -
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
- Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
- Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
- Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
- Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
- Product Citations
-
- Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
- McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
- Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
- Sebastian M, et al. 2016. J Immunol. 196: 144 - 155. PubMed
- de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
- Lee GJ, et al. 2020. Oncoimmunology. 9:1746553. PubMed
- Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
- Yang Z, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.04.035. PubMed
- He C, et al. 2022. Nat Commun. 13:5459. PubMed
- Liu Y, et al. 2019. Nat Commun. 10:324. PubMed
- Laczkó D, et al. 2020. Immunity. 53:724. PubMed
- Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
- Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
- Park JS, et al. 2022. J Transl Med. 20:85. PubMed
- Li X, et al. 2021. Front Immunol. 12:779560. PubMed
- RRID
- AB_2563284 (BioLegend Cat. No. 144611) AB_2563285 (BioLegend Cat. No. 144612)
- Structure
- 35 kD
- Distribution
-
Germinal center B cells, activated B and T cells
- Function
- Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
- Ligand/Receptor
- Neu5Ac-recognizing lectins
- Cell Type
- B cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.
- Gene ID
- NA
- UniProt
- View information about GL7 on UniProt.org
