Purified anti-mouse TCR γ/δ Antibody
- 产品名称：
- Purified anti-mouse TCR γ/δ Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118101
- 产品应用：
- 118101
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Díaz NM, et al. 2020. Invest Ophthalmol Vis Sci. 61:37:00. PubMed
- Belle A, et al. 2012. J Immunol. 188:462. PubMed
- Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed
- Waickman AT, et al. 2017. Cytokine. 99:266. PubMed
- Nobs SP, et al. 2021. J Allergy Clin Immunol. . PubMed
- Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
- Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
- ?lvarez-Salamero C, et al. 2020. PLoS Biol. 18:e3000646. PubMed
- Dasgupta D, et al. 2020. Gastroenterology. 159:1487. PubMed
- Shapiro MJ, et al. 2019. J Immunol. 202:2287. PubMed
- Goodall KJ, et al. 2021. J Biol Chem. 297:101141. PubMed
- Park S, et al. 2021. Nat Cell Biol. 23:476. PubMed
- Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
- Yoshizaki T, et al. 2019. Sci Rep. 9:17067. PubMed
- Kasten K, et al. 2010. Infect Immun. 78:4714. PubMed
- Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
- Bittner-Eddy PD, et al. 2020. Front Immunol. 11:677. PubMed
- Cardoso F, et al. 2021. Nature. 597:410. PubMed
- Castro–Dopico T, et al. 2020. Cell Reports. 32(1):107857. PubMed
- Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
- Lopes N, et al. 2021. Nat Immunol. 22:179. PubMed
- Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
- Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
- Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
- Bréart B, et al. 2011. J Exp Med. 208:1267. PubMed
- Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
- Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
- Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
- Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
- Stadanlick J, et al. 2011. J Immunol. 187:664. PubMed
- Ma Z, et al. 2021. Cancer Med. 10:5358. PubMed
- Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
- RRID
- AB_313826 (BioLegend Cat. No. 118101)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
