PerCP/Cyanine5.5 anti-mouse TCR γ/δ An
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse TCR γ/δ An
- 产品类别：
- 抗体
- 产品编号：
- 118117
- 产品应用：
- 118117
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Polese B, et al. 2021. Cell Reports. 36(4):109456. PubMed
- Cibrián D, et al. 2020. J Allergy Clin Immunol. 145:199. PubMed
- Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
- Mikami Y, et al. 2021. Immunity. 54(3):514-525.e6. PubMed
- Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
- Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
- Sanchez-Felipe L, et al. 2021. Nature. 590:320. PubMed
- Kobayashi T, et al. 2019. Cell. 176:982. PubMed
- Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
- Wang Q, et al. 2020. Wound Repair Regen. 28:105. PubMed
- Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
- Boothby IC, et al. 2021. Nature. 599:667. PubMed
- Chen ELY, et al. 2021. Cell Rep. 35:109227. PubMed
- Babb R, et al. 2016. Clin Sci. 130: 697 - 710. PubMed
- He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
- Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
- Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
- Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
- Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
- Jin C, et al. 2019. Cell. 176:998. PubMed
- Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
- Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
- Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
- Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed
- Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Zhang H et al. 2019. Cell Rep. 29(2):391-405 . PubMed
- Iwanami N, et al. 2020. iScience. 23:101260. PubMed
- Mathur AN, et al. 2019. Immunity. 50:655. PubMed
- Schofield L, et al. 2017. BMC Med. 10.1186/s12916-017-0883-8. PubMed
- Wang X, et al. 2022. Elife. 11:. PubMed
- Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed
- Gangoso E, et al. 2021. Cell. 184:2454. PubMed
- Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
- RRID
- AB_10612572 (BioLegend Cat. No. 118117) AB_10612756 (BioLegend Cat. No. 118118)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
