PE/Cyanine7 anti-mouse TCR γ/δ Antibod
- 产品名称：
- PE/Cyanine7 anti-mouse TCR γ/δ Antibod
- 产品类别：
- 抗体
- 产品编号：
- 118124
- 产品应用：
- 118124
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
- Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
- Jong RM, et al. 2022. J Immunol. 208:407. PubMed
- Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
- Lee J, et al. 2020. Gut Microbes. 1:. PubMed
- Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
- Sellau J, et al. 2016. Sci Rep. 6:28058. PubMed
- Babb R, et al. 2016. Clin Sci. 130: 697 - 710. PubMed
- Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
- Du Y, et al. 2022. Nat Commun. 13:231. PubMed
- , et al. 2021. Eur J Immunol. 51:2708. PubMed
- Panea C, et al. 2021. Commun Biol. 4:913. PubMed
- Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
- Bian G, et al. 2020. British Journal of Pharmacology. 178(3):636-653. PubMed
- Riding AM, et al. 2022. iScience. 25:104660. PubMed
- Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
- Giampazolias E, et al. 2021. Cell. . PubMed
- Gubin MM, et al. 2018. Cell. 175:1014. PubMed
- Macagno M, et al. 2014. J Immunol. 192:5434. PubMed
- Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
- Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
- Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
- Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
- Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
- Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
- Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
- Trivedi S, et al. 2020. Elife. 9:00. PubMed
- RRID
- AB_11203530 (BioLegend Cat. No. 118123) AB_11204423 (BioLegend Cat. No. 118124)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
