- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Polese B, et al. 2021. Cell Reports. 36(4):109456. PubMed
- Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
- Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
- Mackel JJ, et al. 2022. Front Cell Infect Microbiol. 12:974175. PubMed
- Naito Y, et al. 2019. American Journal of Physiology-Renal Physiology. 318(1):F238-F247. PubMed
- Akilov O, et al. 2009. J Immunol. 183:7994. PubMed
- Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
- B?ttcher JP, et al. 2018. Cell. 172:1022. PubMed
- Michel M, et al. 2012. Proc Natl Acad Sci U S A. 109:17459. PubMed
- Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
- Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
- Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
- Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
- Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
- Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
- Hutter K, et al. 2020. FEBS J. . PubMed
- Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
- Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed
- Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
- Moon S, et al. 2021. J Exp Med. 218:. PubMed
- Dai R, et al. 2021. Front Immunol. 12:727046. PubMed
- Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
- Enomoto D, et al. 2017. PLoS One.. 10.1371/journal.pone.0183584. PubMed
- Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
- Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
- Chen W, et al. 2018. Biomed Res Int. 2018:5647120. PubMed
- Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
- Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
- Zhang H et al. 2019. Cell Rep. 29(2):391-405 . PubMed
- Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
- Rouse MD, et al. 2020. Front Microbiol. 11:414. PubMed
- Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
- Ryu S, et al. 2021. eLife. 0.416666666666667. PubMed
- Pender M, et al. 2012. Blood. 120:5085. PubMed
- Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
- Gawish R, et al. 2022. Elife. 11:. PubMed
- Zheng QY, et al. 2020. FASEB J. 34:10590. PubMed
- RRID
- AB_313831 (BioLegend Cat. No. 118107) AB_313832 (BioLegend Cat. No. 118108)
