FITC anti-mouse TCR γ/δ Antibody
- 产品名称：
- FITC anti-mouse TCR γ/δ Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118105
- 产品应用：
- 118105
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Polese B, et al. 2021. Cell Reports. 36(4):109456. PubMed
- Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
- Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
- Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
- Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
- Miller S, et al. 2016. Acta Biochim Biophys Sin. 10.1093/abbs/gmw002. PubMed
- Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
- Kobayashi S, et al. 2019. J Immunol. 203:1447. PubMed
- Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
- Lee P, et al. 2014. J Immunol. 192:178. PubMed
- Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
- Koren E, et al. 2022. Nat Commun. 13:4628. PubMed
- Bauer T, et al. 2021. Life (Basel). 11:. PubMed
- Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
- Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
- Agashe VV, et al. 2018. J Immunol. 201:772. PubMed
- Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
- Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
- Soria-Castro R, et al. 2020. Sci Rep. 10:17802. PubMed
- Kim J, et al. 2012. Immunol Lett. 147:34. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Zhang H et al. 2019. Cell Rep. 29(2):391-405 . PubMed
- Baltanás F, et al. 2013. Mol Cell Biol. 33:4562. PubMed
- Yuan W, et al. 2012. Vaccine. 30:2490. PubMed
- Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
- Wang X, et al. 2022. Elife. 11:. PubMed
- Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
- Lai NY, et al. 2020. Cell. 180:33:00. PubMed
- Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed
- Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
- Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
- Zheng QY, et al. 2020. FASEB J. 34:10590. PubMed
- RRID
- AB_313829 (BioLegend Cat. No. 118105) AB_313830 (BioLegend Cat. No. 118106)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
