Brilliant Violet 421™ anti-mouse TCR γ
- 产品名称：
- Brilliant Violet 421™ anti-mouse TCR γ
- 产品类别：
- 抗体
- 产品编号：
- 118119
- 产品应用：
- 118119
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mL?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
- Schneider C, et al. 2018. Cell. 174:271. PubMed
- Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
- Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
- Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
- Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
- Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
- Hiltensperger M, et al. 2021. Nat Immunol. 22:880. PubMed
- Progatzky F, et al. 2021. Nature. 599:125. PubMed
- Schuldt N, et al. 2015. PLoS One. 10: 0145762. PubMed
- Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
- Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
- Chitu V, et al. 2020. Cell Reports. 30(9):3004-3019. PubMed
- Kovacs SB, et al. 2021. STAR Protoc. 2:100244. PubMed
- Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Yoshida H, et al. 2019. Cell. 176:897. PubMed
- Ulu?kan ?, et al. 2020. Cell Reports. 29(4):844-859.e3.. PubMed
- Jolly A, et al. 2022. Cell Rep Methods. 2:100315. PubMed
- Rezende RM, et al. 2019. J Immunol. 203:2621. PubMed
- Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
- Lai NY, et al. 2020. Cell. 180:33:00. PubMed
- Blanco R, et al. 2014. Sci Signal. 2:354. PubMed
- Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
- RRID
- AB_10896753 (BioLegend Cat. No. 118119) AB_2562566 (BioLegend Cat. No. 118120)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
