Biotin anti-mouse TCR γ/δ Antibody
- 产品名称：
- Biotin anti-mouse TCR γ/δ Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118103
- 产品应用：
- 118103
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
- Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
- Thelin MA, et al. 2017. Diabetes. 66:2220. PubMed
- Fujimori S, et al. 2022. Elife. 11:. PubMed
- Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
- Wang T, et al. 2020. Blood Sci. 2:79. PubMed
- Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
- Higa T, et al. 2022. Nat Commun. 13:1500. PubMed
- Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
- He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
- Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
- Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
- Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
- Zeis P, et al. 2020. Immunity. 53:775. PubMed
- Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
- Okamura T, et al. 2009. Proc Natl Acad Sci U S A. 106:13974. PubMed
- Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
- RRID
- AB_313827 (BioLegend Cat. No. 118103)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
