APC Anti-mouse TCR γ/δ Antibody
- 产品名称：
- APC Anti-mouse TCR γ/δ Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118115
- 产品应用：
- 118115
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- C57BL/6J intraepithelial lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Red Laser (633 nm)
- Application Notes
The GL3 antibody has been shown to be useful in identifying γ/δ?T cells by flow cytometry and immunohistochemistry and depleting γ/δ?T cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections2,6, and in vivo depletion of γ/δ?T cells3-5.
- Application References
(PubMed link indicates BioLegend citation) -
- Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
- Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
- Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
- Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
- Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
- Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
- Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed
- Product Citations
-
- Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
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- Sophie Thiemann et al. 2017. Cell host & microbe. 21(6):682-694 . PubMed
- Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
- Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
- Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
- Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
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- Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
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- Guo Y, et al. 2013. Infect Immun . 81:3923. PubMed
- Pandit M, et al. 2021. Eur J Immunol. 51:1461. PubMed
- Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
- Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
- Li J, et al. 2020. Development. . PubMed
- Zhang X, et al. 2021. Front Pharmacol. 12:629513. PubMed
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- Pai M, et al. 2016. JPEN J Parenter Enteral Nutr. 40: 383 - 391. PubMed
- Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
- Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
- Baja?a S, et al. 2022. iScience. 25:103732. PubMed
- Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
- Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
- Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
- Wang X, et al. 2022. Elife. 11:. PubMed
- Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
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- RRID
- AB_1731824 (BioLegend Cat. No. 118115) AB_1731813 (BioLegend Cat. No. 118116)
- Structure
- Ig superfamily, associates with CD3 complex.
- Distribution
-
T cell subset in thymus, intestinal epithelium, peripheral lymphoid tissues and peritoneum, most γ/δ T cells are CD4-/CD8-, some are CD8+.
- Function
- Antigen recognition; γ/δ T cells are thought to play a role in tolerance.
- Ligand/Receptor
- Some bacterial or tumor antigens bound to MHC class I.
- Cell Type
- Epithelial cells, T cells, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Skarstein K, et al. 1995. Immunology. 81:497.
- Harrison LC, et al. 1996. J?Exp?Med. 184:2167.
- Wildner G, et al. 1996. Eur?J?Immunol. 26:2140.
- Brandes M, et al. 2005. Science. 309:264.
- Gene ID
- 110066 View all products for this Gene ID 110067 View all products for this Gene ID
- UniProt
- View information about TCR gamma/delta on UniProt.org
