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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD86 Antibody
产品名称：
Purified anti-mouse CD86 Antibody
产品类别：
抗体
产品编号：
105001
产品应用：
105001
[价格]
规格 价格 库存
50µg ￥ 648 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105051-105056).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  3. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  4. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  5. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  6. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  7. Zhang P, et al. 2007. J Immunol. 178:474. PubMed
  8. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  9. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  10. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  11. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  12. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  13. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  14. Lai JF, et al. 2020. J Allergy Clin Immunol. 146:1406. PubMed
  15. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  17. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  18. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  19. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  20. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  21. Ahmad F, et al. 2021. J Am Heart Assoc. 10:e017524. PubMed
  22. Vander B, et al. 2017. J Cell Biol. 10.1083/jcb.201512012. PubMed
  23. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  24. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  25. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  26. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  27. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  28. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  29. Zhong Z, et al. 2022. Bioact Mater. 10:195. PubMed
  30. Klinger M, et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293:R677. PubMed
RRID
AB_313144 (BioLegend Cat. No. 105001) AB_313145 (BioLegend Cat. No. 105002)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

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