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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse CD86 Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse CD86 Antibody
产品类别：
抗体
产品编号：
105027
产品应用：
105027
[价格]
规格 价格 库存
25µg ￥ 1416 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS-activated CBA/Ca mouse splenic B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining of acetone-fixed frozen sections2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105051-105056).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
  2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
  5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
  6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
  11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed
Product Citations
  1. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  2. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  3. Szulc-D&acedil;browska L, et al. 2017. PLoS One. 12(6):e0179166. PubMed
  4. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  5. Ortiz G, et al. 2020. Front Immunol. 11:1713. PubMed
  6. Zhu J, et al. 2016. Sci Rep. 6:27136. PubMed
  7. Franks SE, et al. 2019. J Immunol. 202:3381. PubMed
  8. Hou H, et al. 2021. Front Cell Dev Biol. 9:737003. PubMed
  9. Wang H, et al. 2019. Nat Commun. 10:1898. PubMed
  10. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  11. Ribeiro-Gomes F, et al. 2015. Cell Death Dis. 6: 2018. PubMed
  12. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  13. Ise W, et al. 2018. Immunity. 48:702. PubMed
  14. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  15. Nikolos F, et al. 2022. Nat Commun. 13:1487. PubMed
  16. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  17. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  18. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  19. Yang X, et al. 2022. Front Immunol. 13:856230. PubMed
  20. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  21. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  22. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  23. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  24. Raso F, et al. 2018. J Clin Invest. 128:4163. PubMed
  25. Siolas D, et al. 2021. Cell Reports. 36(8):109578. PubMed
  26. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  27. Denieffe S, et al. 2013. Brain Behav Immun. 34:86. PubMed
  28. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
  29. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
  30. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
RRID
AB_893420 (BioLegend Cat. No. 105027) AB_2074994 (BioLegend Cat. No. 105028)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

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