Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

The GL-1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1). Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemical staining of acetone-fixed frozen sections^2,6, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses^1-6. GL-1 is not suitable for immunohistochemical staining of formalin-fixed paraffin sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 105051-105056).

1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
11. de Verteuil DA, et al. 2014. J Immunol. 193:1121. PubMed

1. Joly M, et al. 2014. J Immunol. 193:3947. PubMed
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5. Thwe PM et al. 2017. Cell metabolism. 26(3):558-567 . PubMed
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7. Shukla A, et al. 2021. Adv Nanobiomed Res. 1:. PubMed
8. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
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12. Xiao Y, et al. 2018. Int Immunol. 30:457. PubMed
13. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
14. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science 262:905.
3. Freeman GJ, et al. 1993. Science 262:907.
4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.