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PerCP anti-mouse CD4 Antibody
产品名称：
PerCP anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100431
产品应用：
100431
[价格]
规格 价格 库存
25µg ￥ 1272 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.?For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.?Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. O'Malley G, et al. 2018. Cancer Immunol Res. 1.240277778. PubMed
  2. Zelenka T, et al. 2022. Nat Commun. 13:6954. PubMed
  3. Jiang Q, et al. 2022. Theranostics. 12:59. PubMed
  4. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  5. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  6. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  7. Zhao J, et al. 2022. J Nanobiotechnology. 20:62. PubMed
  8. Blonska M, et al. 2013. Sci Signal. 306:110. PubMed
  9. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  10. Guo X, et al. 2020. Nat Commun. 11:2177. PubMed
  11. Wang Z, et al. 2020. Autophagy. 1:. PubMed
  12. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  13. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  14. Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed
  15. Probst P, et al. 2017. Cancer Res. 77:3644. PubMed
  16. Marks KE, et al. 2021. Cell Reports. 35(13):109303. PubMed
  17. Chen Q, et al. 2016. Nat Commun. 7:13193. PubMed
  18. Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed
  19. Yi X, et al. 2020. Sci Adv. 6:eaba3546. PubMed
  20. Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed
  21. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  22. Zhu W, et al. 2019. FASEB J. 33:5208. PubMed
  23. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  24. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  25. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  26. Yi H, et al. 2021. Front Immunol. 12:719189. PubMed
  27. Geng J, et al. 2014. Biochim Biophys Acta. 1842:1770. PubMed
  28. Cecchinato V, et al. 2017. J Immunol. 198(1):184-195. PubMed
  29. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  30. Zhang J, et al. 2018. Oncoimmunology. 7:e1461301. PubMed
  31. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  32. Aulova KS, et al. 2022. Molecules. 27:. PubMed
  33. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  34. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  35. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  36. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  37. Cao Y, et al. 2022. Small. 18:e2203466. PubMed
  38. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID
AB_893329 (BioLegend Cat. No. 100431) AB_893323 (BioLegend Cat. No. 100432)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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