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Brilliant Violet 510™ anti-mouse CD4 Antibod
产品名称：
Brilliant Violet 510™ anti-mouse CD4 Antibod
产品类别：
抗体
产品编号：
100449
产品应用：
100449
[价格]
规格 价格 库存
50µg ￥ 3288 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.?Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  2. Oetjen LK et al. 2017. Cell. 171(1):217-228 . PubMed
  3. Mancini M, et al. 2019. J Immunol. 202:1479. PubMed
  4. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  5. Chen JC et al. 2018. Cell systems. 7(1):92-103 . PubMed
  6. Saito S, et al. 2020. Nutrients. 12:. PubMed
  7. Shi L, et al. 2021. Immunity. . PubMed
  8. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  9. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  10. Ng KK et al. 2018. eLife. 7 pii: e37851. PubMed
  11. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  12. Verghese DA, et al. 2018. JCI Insight. 3:. PubMed
  13. Galvani E, et al. 2020. Nat Commun. 11:853. PubMed
  14. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  15. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  16. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  17. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  18. Moguche AO et al. 2017. Cell host & microbe. 21(6):695-706 . PubMed
  19. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  20. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  21. Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed
  22. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  23. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  24. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  25. Uche UU, et al. 2018. J Exp Med. 215:3165. PubMed
  26. Hollern DP, et al. 2020. Cell. 179(5):1191-1206.e21.. PubMed
  27. Tomaru U et al. 2019. Cell reports. 26(3):639-651 . PubMed
  28. Sheng WS, et al. 2019. Pain Res Manag. 2019:1260353. PubMed
  29. Smith LK, et al. 2021. Elife. 10:. PubMed
  30. Chavez JS, et al. 2022. Cells. 11:. PubMed
  31. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  32. Goddery EN, et al. 2021. Front Immunol. 12:726421. PubMed
RRID
AB_2564587 (BioLegend Cat. No. 100449)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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