热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
APC/Cyanine7 anti-mouse CD4 Antibody
产品名称：
APC/Cyanine7 anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100413
产品应用：
100413
[价格]
规格 价格 库存
25µg ￥ 1044 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.?Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  2. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  3. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  4. Bergot AS, et al. 2020. J Immunol. 204:1787. PubMed
  5. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  6. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  7. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  8. Li H, et al. 2022. Cell Rep Med. 3:100554. PubMed
  9. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  10. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  11. Dong L, et al. 2021. Cancer Cell. . PubMed
  12. Shao B, et al. 2018. Mol Med Rep. 18:920. PubMed
  13. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  14. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  15. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  16. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  17. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  18. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  19. Wang Q, et al. 2020. Wound Repair Regen. 28:105. PubMed
  20. Saito S, et al. 2020. Nutrients. 12:. PubMed
  21. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  22. Wang X, et al. 2021. Front Cell Dev Biol. 632805:9. PubMed
  23. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  24. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  25. Treger RS, et al. 2020. Immunity. 50(2):334-347.e9.. PubMed
  26. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  27. Tu X, et al. 2022. Nat Commun. 13:6977. PubMed
  28. Ciarlo E, et al. 2016. Sci Rep. 6:37944. PubMed
  29. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  30. Ontiveros-Padilla L, et al. 2021. Microorganisms. 9:. PubMed
  31. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  32. Collinson-Pautz M, et al. 2016. PLoS One. 11:e0164547. PubMed
  33. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  34. Yu X, et al. 2022. J Extracell Vesicles. 11:e12185. PubMed
  35. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  36. Feng H, et al. 2020. J Dermatol. 47:147. PubMed
  37. Levi J, et al. 2020. J Nucl Med. . PubMed
  38. Poczobutt J, et al. 2016. J Immunol. 196: 891 - 901. PubMed
  39. Holvoet B, et al. 2015. Stem Cell Reports. 5: 1183-1195. PubMed
  40. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  41. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  42. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  43. Levescot A, et al. 2021. J Clin Invest. 131:. PubMed
  44. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  45. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  46. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  47. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  48. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  49. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  50. St Clair JB, et al. 2017. PLoS One. 12:e0170556. PubMed
  51. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
  52. Hum NR, et al. 2022. Front Oncol. 12:928474. PubMed
  53. Mara AB, et al. 2022. NPJ Vaccines. 7:86. PubMed
  54. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  55. Zhang L, et al. 2021. Clin Transl Med. 11:e464. PubMed
  56. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  57. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  58. Kr?utler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  59. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  60. Jorapur A, et al. 2022. PLoS Pathog. 18:e1010200. PubMed
  61. Dey S, et al. 2020. J Immunother Cancer. 8:. PubMed
  62. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  63. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  64. Brown SM et al. 2017. Endocrinology. 158(10):3592-3604 . PubMed
  65. Sydney Lavoie et al. 2019. eLife. 8 pii: e39982. PubMed
  66. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  67. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  68. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  69. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  70. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  71. Merlo LM, et al. 2020. Clin Pathol. 13:2632010X20951812. PubMed
  72. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  73. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  74. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  75. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  76. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  77. Li M, et al. 2021. Cancers (Basel). 13:. PubMed
  78. Dong X, et al. 2022. Front Immunol. 13:896472. PubMed
  79. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  80. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  81. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  82. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  83. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  84. McNamee E, et al. 2013. Gut. 62:53. PubMed
  85. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  86. Wang X, et al. 2021. Cell. 184:5357. PubMed
  87. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  88. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  89. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  90. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  91. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  92. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  93. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  94. Le QV, et al. 2022. Bioact Mater. 15:160. PubMed
  95. Ma C, et al. 2021. J Exp Med. :218. PubMed
  96. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  97. Sheng J, et al. 2021. eLife. 10:00. PubMed
  98. Chen S, et al. 2015. Cancer Res . 7: 519-531. PubMed
  99. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  100. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  101. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  102. Chen B, et al. 2021. Cell. 184:6262. PubMed
  103. Wang X, et al. 2022. Elife. 11:. PubMed
  104. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  105. Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
  106. Li A, et al. 2018. Cancer Lett. 431:54. PubMed
  107. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  108. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  109. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  110. Ma C, et al. 2020. Sci Adv. 6:eaaz6717. PubMed
  111. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  112. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  113. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
  114. Liang Z, et al. 2022. iScience. 25:105233. PubMed
  115. Li H, et al. 2021. Signal Transduct Target Ther. 6:389. PubMed
  116. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_312698 (BioLegend Cat. No. 100413) AB_312699 (BioLegend Cat. No. 100414)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线