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Alexa Fluor® 700 anti-mouse CD4 Antibody
产品名称：
Alexa Fluor® 700 anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100429
产品应用：
100429
[价格]
规格 价格 库存
25µg ￥ 1128 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.


* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.?Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  2. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  3. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  4. Denk D, et al. 2022. Immunity. 55:2059. PubMed
  5. Qi Z, et al. 2022. Nat Commun. 13:182. PubMed
  6. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  7. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  8. Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed
  9. Alotaibi F, et al. 2021. Front Immunol. 11:584937. PubMed
  10. Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed
  11. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  12. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  13. García Nores GD, et al. 2018. Nat Commun. 9:1970. PubMed
  14. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  15. Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed
  16. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  17. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  18. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  19. Yap JY, et al. 2018. Immunology. 154:522. PubMed
  20. Sharba S, et al. 2019. Virulence. 10:97. PubMed
  21. Bharath LP, et al. 2020. Cell Metabolism. 32(1):44-55.e6.. PubMed
  22. Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed
  23. Yuan Y, et al. 2022. Cell Rep. 38:110256. PubMed
  24. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  25. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  26. Araujo Furlan CL, et al. 2018. Front Immunol. 2.149305556. PubMed
  27. Zhang P, et al. 2022. Nat Commun. 13:1588. PubMed
  28. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
  29. Brown ZJ, et al. 2018. Cell Death Dis. 0.805555556. PubMed
  30. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  31. Panagi M, et al. 2020. Theranostics. 10:1910. PubMed
  32. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  33. Altin J, et al. 2014. Proc Natl Acad Sci U S A. 111:2067. PubMed
  34. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  35. van Loon K, et al. 2022. Cancers (Basel). 14:. PubMed
  36. Barrigan L, et al. 2013. Infect Immun . 81:2028. PubMed
  37. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  38. Szeto C, et al. 2022. Nat Commun. 13:4951. PubMed
  39. Gawish R, et al. 2022. Elife. 11:. PubMed
  40. Stienne C, et al. 2022. Cell Rep. 38:110553. PubMed
  41. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
RRID
AB_493698 (BioLegend Cat. No. 100429) AB_493699 (BioLegend Cat. No. 100430)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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