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Alexa Fluor® 488 anti-mouse CD4 Antibody
产品名称：
Alexa Fluor® 488 anti-mouse CD4 Antibody
产品类别：
抗体
产品编号：
100425
产品应用：
100425
[价格]
规格 价格 库存
25µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F?- Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5-5 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.?Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  13. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Parlane N, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  2. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  3. Barberio AE, et al. 2020. ACS Nano. 14:11238. PubMed
  4. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  5. Onishi S, et al. 2015. PLoS One. 10:126564. PubMed
  6. Porsche CE, et al. 2021. JCI Insight. 6:. PubMed
  7. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  8. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  9. Inoue M, et al. 2014. Proc Natl Acad Sci U S A. 111:5295. PubMed
  10. Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed
  11. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  12. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  13. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  14. Namai F, et al. 2020. Exp Mol Med. 52:1627. PubMed
  15. Meyer LK, et al. 2019. J Clin Invest. 130:863. PubMed
  16. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  17. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  18. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  19. Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed
  20. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  21. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  22. Collins N, et al. 2016. Nat Commun. 7:11514. PubMed
  23. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  24. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  25. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
  26. Bajic D, et al. 2020. Mol Cancer Ther. 19:2554. PubMed
  27. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  28. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  29. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  30. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  31. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  32. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  33. Park JS, et al. 2022. J Transl Med. 20:85. PubMed
RRID
AB_493520 (BioLegend Cat. No. 100425) AB_389302 (BioLegend Cat. No. 100423)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

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