Purified anti-human CD163 Antibody
- 产品名称：
- Purified anti-human CD163 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 333602
- 产品应用：
- 333602
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation,?western blot1, and spatial biology (IBEX)6,7.
- Application References
(PubMed link indicates BioLegend citation) -
- Pulford K,?et al.?1992.?Immunology?75:588. (ICC, IP, WB)
- Law SK,?et al.?1993.?Eur. J. Immunol.?23:2320.
- Madsen M,?et al.?2004.?J. Biol. Chem.?279:51561.
- Kim WK,?et al.?2006.?Am. J. Pathol.?168:822. (FC)
- Buttari B,?et al.?2011.?Atherosclerosis.?215:316.?PubMed
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
- Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
- Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
- Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed
- Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
- Syrimi E, et al. 2021. iScience. 24:103215. PubMed
- Hu G, et al. 2021. Nat Commun. 12:773. PubMed
- Greven D, et al. 2015. Ann Rheum Dis. 74: 1924 - 1930. PubMed
- Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed
- Kim J, et al. 2022. Sci Rep. 12:7753. PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
- Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed
- Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
- Keskin DB, et al. 2019. Nature. 565:234. PubMed
- Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
- Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
- RRID
- AB_1088991 (BioLegend Cat. No. 333602)
- Structure
- 134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
- Distribution
-
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
- Function
- Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
- Ligand/Receptor
- Haptoglobin-hemoglobin complex, TWEAK
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179
- Gene ID
- 9332 View all products for this Gene ID
- UniProt
- View information about CD163 on UniProt.org
