PerCP/Cyanine5.5 anti-human CD163 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD163 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 333607
- 产品应用：
- 333607
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation,?western blot1, and spatial biology (IBEX)6,7.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Pulford K,?et al.?1992.?Immunology?75:588. (ICC, IP, WB)
- Law SK,?et al.?1993.?Eur. J. Immunol.?23:2320.
- Madsen M,?et al.?2004.?J. Biol. Chem.?279:51561.
- Kim WK,?et al.?2006.?Am. J. Pathol.?168:822. (FC)
- Buttari B,?et al.?2011.?Atherosclerosis.?215:316.?PubMed
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
- Bsat M, et al. 2021. Curr Protoc. 1:e74. PubMed
- Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
- Bekere I, et al. 2021. PLoS Pathog. 17:e1010074. PubMed
- Izadi D, et al. 2019. Sci Adv. 5:eaay0370. PubMed
- Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
- Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
- Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
- Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
- Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
- Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed
- Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
- Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
- Zhang Z, Shively J 2010. PLoS One. 5:e13985. PubMed
- Ohmatsu H, et al. 2017. Oncoimmunology. 6:e1181237. PubMed
- Ramaseri Sunder S, et al. 2020. J Interferon Cytokine Res. 40:310. PubMed
- Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
- Campbell J, et al. 2014. PLoS Pathog. 10:1004533. PubMed
- RRID
- AB_1134006 (BioLegend Cat. No. 333607) AB_2228986 (BioLegend Cat. No. 333608)
- Structure
- 134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
- Distribution
-
Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells
- Function
- Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
- Ligand/Receptor
- Haptoglobin-hemoglobin complex, TWEAK
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179
- Gene ID
- 9332 View all products for this Gene ID
- UniProt
- View information about CD163 on UniProt.org
