Purified anti-mouse CD326 (Ep-CAM) Antibody
- 产品名称：
- Purified anti-mouse CD326 (Ep-CAM) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118201
- 产品应用：
- 118201
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TE-71 thymic epithelial cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, ICC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of?frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.
- Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
- Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
- Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
- Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Ding X, et al. 2015. Cancer Res. 75:330. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Thorsen AS, et al. 2020. Dis Model Mech. 14:dmm046706. PubMed
- Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
- Huang L, et al. 2020. JCI Insight. 5:00. PubMed
- Nudel I, et al. 2011. J Immunol. 186:891. PubMed
- Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
- Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
- Karabacak NM, et al. 2022. Cancer Res. 82:1084. PubMed
- Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
- Foster DS, et al. 2020. Nat Commun. 3.278472222. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
- Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
- Liu H, et al. 2020. J Immunol. 205:1207. PubMed
- Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
- Fuhrmann L, et al. 2021. Int J Mol Sci. 22:. PubMed
- E-Lacerda RR, et al. 2020. Clin Exp Allergy. 256:50. PubMed
- Corcos N, et al. 2021. Frontiers in Immunology. 12:616215. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Daley D, et al. 2017. Nat Med. 23:556. PubMed
- Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
- Billings SE, et al. 2021. Development. 148: . PubMed
- Thorsen AS, et al. 2020. Dis Model Mech. . PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Tomchaney M, et al. 2021. BMC Pulm Med. 21:275. PubMed
- Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
- Yamamoto K, et al. 2022. STAR Protoc. 3:101155. PubMed
- Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
- Molina LM, et al. 2021. Cell Reports. 36(1):109310. PubMed
- Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
- Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
- Burger ML, et al. 2021. Cell. 184:4996. PubMed
- Iyer N, et al. 2020. PLoS Pathog. 16:e1008360. PubMed
- Van Winkle JA, et al. 2020. J Virol. 94:. PubMed
- Zhou Y, et al. 2020. Am J Pathol. 190:1414. PubMed
- Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
- RRID
- AB_1089026 (BioLegend Cat. No. 118201) AB_1089027 (BioLegend Cat. No. 118202)
- Structure
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Distribution
-
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
- Function
- Mediates calcium-independent homophilic cell-cell adhesion.
- Interaction
- CD326 displays hemophilic binding.
- Ligand/Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
- Gene ID
- 17075 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
